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Item #:
90792252
PBS [10X] (Phosphate Buffered Saline), 1 US Gallon
83% of respondents would recommend this to a friend
Item #:
90792252
JMD 58365
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10X PBS is a sterile and ready-to-use solution that simplifies your experiments.
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What Stands Out
Premium Quality
PBS [10X] is formulated with high-purity reagents ensuring consistent performance in laboratory settings, supporting reliable experimental results crucial for scientists and researchers.
Large Volume
Offering 1 US gallon, this bulk supply is ideal for extensive laboratory applications and minimizes the need for frequent replenishment, enhancing convenience and cost-effectiveness.
Versatile Application
This PBS [10X] solution is compatible with various biological protocols, including cell culture and immunostaining, making it an essential buffer for diverse research needs in biomedical fields.
Product Details
| Item Form | Liquid |
| Volume | 1 gallons |
| Brand Name | G BIOSCIENCES |
| Manufacturer | G-Biosciences |
| Part Number | R028 |
| Brand | G BIOSCIENCES |
| Item Volume | 1 gallons |
| Item Weight | 8.34 lbs (3.78 kg) |
Who Should Buy?
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Laboratory Researchers
Ideal for researchers needing a reliable buffer solution for various biological and chemical experiments in laboratories.
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Cell Culture Work
Perfect for maintaining cell viability and supporting the growth requirements of various cell lines in culture.
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Clinical Applications
Useful for diagnostic and therapeutic applications, ensuring stable pH and ion concentrations in biological samples.
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Home Use
Not suitable for casual home experiments, as it's a specialized product intended for laboratory or clinical use.
Product Description
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Customer Questions & Answers
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Question:
What is PBS [10X] (Phosphate Buffered Saline) used for?
Answer: PBS [10X] is primarily used in biological and biochemical applications. It's a buffer solution that helps maintain the pH and osmolarity of biological materials, which is crucial for experiments involving cell culture, molecular biology, and protein research. For instance, it can be utilized to wash cells before analysis or as a diluent for biochemical assays, ensuring consistent and reliable results. -
Question:
How do you prepare working solutions from PBS [10X]?
Answer: To prepare a working solution from PBS [10X], simply dilute the concentrate with distilled water to achieve a 1X solution. For example, mix one part of PBS [10X] with nine parts of distilled water. This method is convenient for customization in various experimental protocols, enabling flexibility in adjusting the volume according to your needs while ensuring optimal conditions for your experiments. -
Question:
Is PBS [10X] suitable for cell culture applications?
Answer: Yes, PBS [10X] is commonly used in cell culture applications. It provides a stable pH environment and isotonic conditions, crucial for maintaining cell viability during procedures like trypsinization and washing. Using PBS helps to remove serum proteins that may interfere with downstream applications, ensuring that cells remain healthy and functional for research and experimentation. -
Question:
Can PBS [10X] be used for protein purification?
Answer: Absolutely, PBS [10X] is often employed in protein purification protocols. Its buffering capacity helps maintain the stability of proteins during the precipitation, centrifugation, and dialysis steps. This versatility is essential in studies where the purity of protein samples is paramount, such as in Western blotting or enzyme activity assays. -
Question:
What are the storage conditions for PBS [10X]?
Answer: PBS [10X] should ideally be stored at room temperature, away from direct sunlight to prevent degradation of its components. For longer-term storage, refrigeration is preferred. Proper storage preserves its buffering capacity and ensures its effectiveness in experiments, making it a reliable choice for researchers needing high-quality reagents. -
Question:
Is there a difference between PBS [10X] and PBS [1X]?
Answer: Yes, the primary difference lies in their concentrations. PBS [10X] is a concentrated solution that must be diluted to create PBS [1X] for use in experiments. While PBS [10X] can be stored for longer periods, PBS [1X] is ready-to-use in biological applications, making it essential to prepare it correctly to maintain the buffer's effectiveness for dependable results. -
Question:
Can PBS [10X] be used in immunoassays?
Answer: Yes, PBS [10X] is frequently utilized in various immunoassays due to its neutral pH and isotonicity, which prevent non-specific binding and maintain antibody stability. This makes it ideal for ELISA and other immunoassay protocols where accurate detection and quantification of antigens or antibodies are crucial for research outcomes. -
Question:
What is the pH of PBS [10X]?
Answer: PBS [10X] has a pH range typically between 7.2 and 7.4. This neutral pH is vital for maintaining physiological conditions for many biological experiments, ensuring minimal impact on cellular processes. This characteristic makes it an optimal buffer for a wide range of applications, from cell biological studies to enzyme kinetics. -
Question:
Where can I buy PBS [10X] (Phosphate Buffered Saline), 1 US Gallon in Jamaica?
Answer: You can conveniently purchase PBS [10X] (Phosphate Buffered Saline), 1 US Gallon through Ubuy in Jamaica. Ubuy offers a straightforward shopping experience, allowing you to find this essential laboratory reagent along with other supplies you may need for your research or experiments.
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JMD 58365
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Features & Benefits
- 10-fold concentrated sterile solution.
- Easily prepares PBS by diluting 1 to 10 in ultrapure water.
- Provides a pH of 7.4 for optimal results.
- Contains essential components like NaH2PO4, NaCl, KCl, and KH2PO4.
- Conveniently sterile filtered through a 0.2µm filter.
- Ideal for various laboratory and research applications.
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