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Item #:
53994661
Agarose, Molecular Biology Grade 100G, P05-SR01-100
Item #:
53994661
JMD 40947
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Rx Biosciences Molecular Biology Grade Agarose is highly purified agarose with very low EEO values certified by strict quality control test procedures.
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What Stands Out
High Purity
This agarose is molecular biology grade, ensuring high purity for reliable results in gel electrophoresis and other applications critical for accurate scientific research.
Versatile Applications
Ideal for various molecular biology techniques, this agarose supports DNA/RNA separation, cloning, and more, making it a staple in any laboratory focused on genetic research.
Bulk Packaging
Packaged in 100 grams, this agarose provides an economical solution for labs needing consistent supply, reducing the frequency of reorders and ensuring uninterrupted research workflows.
Product Details
| Item Weight | 0.22 lbs (100 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for those conducting DNA/RNA analysis and gel electrophoresis for high-resolution separation.
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Academic Researchers
Suitable for academic labs conducting experiments involving gene cloning, sequencing, and other molecular biology applications.
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Laboratory Educators
Useful for educators in teaching molecular biology techniques and providing hands-on experience with gel electrophoresis.
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Non-Biological Fields
Not suitable for users outside of biological research, such as engineering or physical sciences.
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Home Experiments
Not recommended for casual home experiments due to the specific requirements of handling and processing agarose.
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Large Scale Production
Not ideal for industries needing bulk agarose supplies; the 100g package is limited for extensive production use.
Product Description
Agarose, Molecular Biology Grade 100G, P05-SR01-100
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Customer Questions & Answers
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Question:
What is Agarose, Molecular Biology Grade?
Answer: Agarose, Molecular Biology Grade, is a purified polysaccharide derived from red algae, primarily used as a gel matrix in molecular biology applications. It provides a stable platform for the separation of DNA, RNA, and proteins based on size during electrophoresis. The high purity ensures minimal background interference, allowing precise separation and accurate results in experiments such as gel electrophoresis and Southern blotting. -
Question:
What are the common uses of Agarose in molecular biology?
Answer: Agarose is commonly used in techniques such as gel electrophoresis for the separation and analysis of nucleic acids and proteins. It is also utilized in techniques like pulsed-field gel electrophoresis for analyzing larger DNA fragments and in the preparation of molecular sieving gels. Its versatility makes it essential for cloning, sequencing, and other genomic research applications. -
Question:
How do I prepare Agarose gel?
Answer: To prepare an Agarose gel, start by weighing the desired amount of Agarose powder (e.g., 1g for a 1% gel) and dissolve it in a buffer solution (like TAE or TBE) by heating until fully melted. Once cooled to approximately 60°C, pour the gel solution into a casting tray and insert a comb to form wells. Allow the gel to solidify for about 30 minutes before using it for electrophoresis. -
Question:
Is Agarose safe to handle in the lab?
Answer: Yes, Agarose is considered safe for use in laboratory settings. It is non-toxic and does not pose significant health risks when handled. However, standard laboratory safety protocols should always be followed, such as wearing gloves and goggles, especially when preparing and heating the Agarose solution to prevent burns and allergic reactions. -
Question:
What is the significance of molecular biology grade Agarose?
Answer: Molecular biology grade Agarose indicates a high level of purity, specifically processed to eliminate contaminants that could interfere with results. This grade ensures that the Agarose does not affect the migration of nucleic acids or proteins during electrophoresis, leading to accurate band sizes and valid conclusions in experimental protocols. -
Question:
Can I reuse Agarose gels?
Answer: Reuse of Agarose gels is generally not recommended as their electrochemical properties may degrade after one use. Repeated runs can also introduce contaminants that affect the clarity and accuracy of results. For best practices, it’s recommended to prepare fresh gels for each experiment, ensuring optimal performance and reproducibility. -
Question:
How do I store Agarose?
Answer: Agarose powder should be stored in a cool, dry place away from moisture and direct sunlight to maintain stability. Once prepared as a gel, it can be stored at 4°C for a short period; however, prolonged storage should ideally be avoided to maintain its integrity. If longer storage is needed, it is better to store the gel in a buffer solution to prevent dehydration. -
Question:
What is the shelf life of Agarose, Molecular Biology Grade?
Answer: The shelf life of Agarose, Molecular Biology Grade, is typically about 2 to 3 years when stored properly in a cool, dry environment. However, factors like temperature and humidity can affect its longevity. Always check the packaging for an expiration date and perform quality checks, such as gel clarity and consistency, before use, particularly for time-sensitive applications. -
Question:
Can I use Agarose for protein gel electrophoresis?
Answer: Agarose can be used for protein gel electrophoresis, especially for the separation of larger proteins or in native conditions. While SDS-PAGE is more common for smaller proteins, using Agarose allows for the maintenance of protein structure and function during electrophoresis, making it suitable for specific applications like enzyme assays or protein retention studies. -
Question:
Where can I buy Agarose, Molecular Biology Grade 100G, P05-SR01-100?
Answer: You can buy Agarose, Molecular Biology Grade 100G, P05-SR01-100 at Ubuy. They offer a wide range of laboratory supplies and equipment, making it a reliable platform for obtaining high-quality Agarose for your molecular biology needs.
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JMD 40947
Order now and get it around Sunday, July 12
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Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Features & Benefits
- - Optimal concentration between 0.5 to 4% in all typical buffer systems
- - Suitable for resolving RNA, DNA and small nucleotides
- - Highly purified agarose
- - Very low EEO values
- - Certified by strict quality control test procedures