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Item #:
87929333
PR1000-500, Taq DNA Polymerase, 500 Reactions (2 Packs of 500 pcs)
Item #:
87929333
JMD 28128
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What Stands Out
High Efficiency
PR1000-500 Taq DNA Polymerase offers superior amplification efficiency, ensuring reliable results across 500 reactions, making it ideal for researchers needing consistent performance in their experiments.
Cost-Effective
With two packs containing 500 reactions each, this product provides exceptional value, reducing per-reaction costs significantly, perfect for budget-conscious laboratories without compromising quality.
Versatile Usage
Designed for a wide range of applications, including cloning and sequencing, the PR1000-500 adapts easily to various protocols, addressing the needs of diverse research projects.
Product Details
| Item Weight | 2 lbs (910 grams) |
Who Should Buy?
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Molecular Biology Labs
Ideal for labs conducting routine PCR amplifications requiring high fidelity and reliable reaction outcomes for research.
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Educational Institutions
Perfect for teaching institutions needing bulk supplies for student experimentation in genetics and molecular biology courses.
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Research Projects
Beneficial for researchers needing extensive numbers of reactions for large-scale experiments while maintaining accuracy and efficiency.
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Casual Users
Less suitable for hobbyists or casual users who may require small quantities of polymerase for limited experiments.
Product Description
PR1000-500, Taq DNA Polymerase, 500 Reactions (2 Packs of 500 pcs)
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Customer Questions & Answers
-
Question:
What is Taq DNA Polymerase and how is it used?
Answer: Taq DNA Polymerase is a thermostable enzyme used for DNA amplification during polymerase chain reaction (PCR) processes. It withstands high temperatures, making it optimal for cyclic denaturation and annealing stages of PCR. It’s essential for cloning, sequencing, and other molecular biology applications, allowing researchers to replicate specific DNA sequences efficiently. -
Question:
What types of applications is the PR1000-500 Taq DNA Polymerase suitable for?
Answer: The PR1000-500 Taq DNA Polymerase is ideal for a range of applications, including standard PCR, RT-PCR, and quantitative PCR. Whether you're working on cloning, genotyping, or gene expression assays, this enzyme is versatile enough to meet your research needs. Its strong performance across various protocols makes it a go-to choice for molecular biology labs. -
Question:
How should I store PR1000-500 Taq DNA Polymerase?
Answer: The PR1000-500 Taq DNA Polymerase should be stored at -20°C for long-term use. This storage condition ensures enzyme stability and activity. Always avoid multiple freeze-thaw cycles, as this can reduce enzyme efficacy. For practical use, it's advisable to aliquot the enzyme into smaller portions, allowing you to thaw only what you need for each experiment. -
Question:
Can I use PR1000-500 Taq DNA Polymerase for high-fidelity applications?
Answer: While Taq DNA Polymerase is excellent for standard PCR, it is not engineered for high-fidelity applications. For superior accuracy, consider using a high-fidelity polymerase designed for precise amplification. However, if you need rapid amplification without stringent fidelity requirements, PR1000-500 can still perform efficiently. -
Question:
What are the benefits of using PR1000-500 Taq DNA Polymerase?
Answer: Using PR1000-500 Taq DNA Polymerase offers several benefits, including robust performance in routine PCR, high tolerance to contamination, and ease of use in diverse applications. It’s reliable for amplifying templates with low copy numbers and helps streamline workflows in molecular biology research, allowing scientists to achieve reproducible results. -
Question:
What are the optimal PCR conditions for PR1000-500 Taq DNA Polymerase?
Answer: Optimal PCR conditions using PR1000-500 Taq DNA Polymerase typically involve an initial denaturation step at 94°C, followed by 25-35 cycles of denaturation (94°C), annealing (50-65°C depending on primers), and extension (72°C). Following amplification, a final extension step of 5-10 minutes at 72°C is recommended for full product length. These settings help ensure efficient DNA amplification. -
Question:
How reliable is PR1000-500 Taq DNA Polymerase for reproducibility?
Answer: PR1000-500 Taq DNA Polymerase is well-regarded for its reliability and reproducibility in PCR assays. Its robust nature enables consistent results across multiple experiments, making it a trusted choice for researchers looking for dependable DNA amplification. Its performance has been validated in various scientific studies, showcasing its efficiency and accuracy. -
Question:
Is there a specific protocol for using PR1000-500 Taq DNA Polymerase?
Answer: Yes, using PR1000-500 Taq DNA Polymerase is straightforward. Prepare your PCR reaction mix containing template DNA, primers, dNTPs, buffer, and the polymerase itself. Follow your specific protocol regarding temperatures and reaction times. Detailed user manuals and protocols are often included or available online, providing step-by-step instructions tailored for optimal results. -
Question:
Where can I find the PR1000-500, Taq DNA Polymerase, 500 Reactions 2 Packs of 500 pcs in Jamaica?
Answer: You can conveniently purchase the PR1000-500, Taq DNA Polymerase, 500 Reactions 2 Packs of 500 pcs from Ubuy. They offer a reliable platform for obtaining this essential enzyme for your molecular biology projects. -
Question:
What is the shelf life of PR1000-500 Taq DNA Polymerase?
Answer: The shelf life of PR1000-500 Taq DNA Polymerase typically ranges from 1 to 2 years when stored properly at -20°C. Check the specific lot number for its expiration date on the packaging. For best outcomes in your experiments, use the enzyme before this date to ensure maximum enzyme activity.
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Pros
- High efficiency in DNA amplification
- Reliable performance across reactions
- Easy to use for experiments
- Compatible with various templates
- Excellent customer support
Cons
- May require precise temperature control.
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JMD 28128
Order now and get it around Thursday, August 27
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Features & Benefits
- Ideal for routine PCR applications as well as genotyping, colony PCR and fast PCR.
- Improved template affinity and solubility for higher enzyme activity and greater yields.
- Proprietary buffer optimized for a variety of assay conditions.
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